Background.MiR-29a is known as a repressor of human cancer.However, its relevance in glioma proliferation and invasion remains\nlargely unknown. In this study, we aimed to investigate the function and mechanismofmiR-29a in glioma tumorigenesis. Methods.\nThe expression of miR-29a was determined by using qRT-PCR. CCK-8, wound healing, and transwell invasion assays were carried\nout to analyze the effects of miR-29a in glioblastoma cells. qRT-PCR, luciferase reporter, and western blot experiments were done\nto validate the targeting of TRAF4/Akt pathway by miR-29a. The expression correlation between levels of TRAF4 and miR-29a\nwas analyzed. Regulation of miR-29a expression by enhanced/reduced TRAF4/Akt expression was finally confirmed by qRT-PCR.\nResults. MiR-29a was decreased in the glioma tissues, especially in those at higher grades. Following its mimic transfection, we\nvalidated thatmiR-29a inhibited cell proliferation, migration, and invasion. Consistently, miR-29a inhibition induced the opposite\neffects on cell proliferation, migration, and invasion. We confirmed TRAF4 as a direct target of miR-29a, which might mediate\nthe Akt pathway activation. We showed a significantly negative expression correlation between TRAF4 and miR-29a in normal\nand glioma tissues. Finally we observed an upregulation of miR-29a in TRAF4/Akt activated cells. Conclusion. MiR-29a is critical\ntumor suppressor for glioma tumorigenesis by forming a negative feedback loop of TRAF4/Akt signaling and represents a potent\ntherapeutic candidate for treating gliomas.
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